產(chǎn)品名稱:MTT噻唑藍(lán) 細(xì)胞檢測
產(chǎn)品型號:M8180
產(chǎn)品特點:MTT噻唑藍(lán) 細(xì)胞檢測貨號:M8180規(guī)格:250mg/支CAS:298-93-1分子式:C18H16N5SBr分子量:414.3儲存條件:2-8℃ 避光純度:>98.0外觀(性狀):黃色粉末單位:瓶
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MTT噻唑藍(lán) 細(xì)胞檢測
貨號:M8180
規(guī)格:250mg/支
說明:
生化研究中酶活力的測定,也可用于細(xì)胞增殖檢測。
特性:純度(%):>98.0 溶解性:溶于甲醇和DMSO,微溶于水
MTT是一種檢測細(xì)胞存活和生長的方法。MTT實驗原理為活細(xì)胞線粒體中的琥珀酸脫氫酶能使外源性MTT還原為水不溶性的藍(lán)紫色結(jié)晶甲瓚(Formazan)并沉積在細(xì)胞中,而死細(xì)胞無此功能。二甲基亞砜(DMSO)能溶解細(xì)胞中的甲瓚,用酶聯(lián)免疫檢測儀在490nm波長處測定其光吸收值,可間接反映活細(xì)胞數(shù)量。
在一定細(xì)胞數(shù)范圍內(nèi),MTT結(jié)晶形成的量與細(xì)胞數(shù)成正比。根據(jù)測得的吸光度值(OD值),來判斷活細(xì)胞數(shù)量,OD值越大,細(xì)胞活性越強(如果是測藥物毒性,則表示藥物毒性越小)。該方法已廣泛用于一些生物活性因子的活性檢測、大規(guī)模的抗腫瘤藥物篩選、細(xì)胞毒性試驗以及腫瘤放射敏感性測定等。它的特點是靈敏度高、經(jīng)濟(jì)。
MTT通常用于.細(xì)胞增殖及細(xì)胞活性測定以及藥物(也包括其他處理方式如放射線照射)對體外培養(yǎng)的細(xì)胞毒性的測定。
缺點:由于MTT經(jīng)還原所產(chǎn)生的甲瓚產(chǎn)物不溶于水,需被溶解后才能檢測。這不僅使工作量增加,也會對實驗結(jié)果的準(zhǔn)確性產(chǎn)生影響,而且溶解甲的有機溶劑對實驗者也有損害。
MTT噻唑藍(lán) 細(xì)胞檢測訂購說明:
1、絕大部分產(chǎn)品備有現(xiàn)貨,一般情況下都能訂貨當(dāng)日發(fā)貨。
2、部分非常用產(chǎn)品,需提前1-2日預(yù)訂,海外期貨則需要提前3-6周預(yù)訂。
3、部分產(chǎn)品價格會因貨期、批次等因素發(fā)生變化,若有變動以訂貨當(dāng)日新價格為準(zhǔn)。
4、每日訂單截止時間為16點整,部分城市可到17點整,因超過截止時間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
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運輸說明:
1、極低溫產(chǎn)品:極低溫產(chǎn)品運輸過程中加裝干冰運輸。用干冰把產(chǎn)品包裹起來,再用泡沫盒密封,膠帶層層粘住泡沫盒,放入索萊寶的箱子,然后交付給合作快遞,安全快速高效放心的送客戶的手中,保證產(chǎn)品的性質(zhì)穩(wěn)定如一,為您的實驗保駕護(hù)航。
2、低溫產(chǎn)品:低溫產(chǎn)品運輸過程中加裝索萊寶冰袋運輸。事先用冰袋把產(chǎn)品包裹起來,再使用泡沫盒密封,用膠帶嚴(yán)實密封泡沫盒,再放入索萊寶的箱子(保溫效果少可以持續(xù)一周),然后交付給合作快遞,安全快速高效放心的送客戶的手中,保證產(chǎn)品的性質(zhì)穩(wěn)定如一,為您的實驗保駕護(hù)航。
3、常溫產(chǎn)品:常溫產(chǎn)品運輸過程中無需加冰或者特殊包裝。產(chǎn)品由公司庫房人員快速配貨,準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。
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